(ASI = SI peak - S I baseline) were calculated and compared by linear regression analysis to coronary artery flow The up slope of the signal intensitytime curves and the maximum signal intensity variation For each region, signal intensity-time curves wereĮxtracted for the whole sequence and for the different levels of coronary flows. Were defined after endocardial and epicardial borders detection.
#Dextran mr contrast software#
With a software dedicated to dynamic image analysis Myocardial signal intensity variations were determined This chemical induced a reversible inhibition of the actinmyosin interaction while maintaining myocardial perfusion. Monoxime (BDM 97% Aldrich, Paris, France), at 30 mmol/ Were arrested by adding to the perfusate 2,3-Butanedione Perfusate was bubbled using an oxygenator (Monolyth (pH 7.4) mixed with fresh blood withdrawn after catheterization of the inferior vena cava in a proportion of 4:1. Introduced into the balloon to delineate the left ventricularĭuring the experiment, the coronary arteries were perfused with a modified Krebs-Henseleit bicarbonate buffer Was cut and a latex balloon was inserted into the left ventricle and sutured on the mitral valve annulus. Proximal left and right coronary arteries were isolated and selectively cannulated. Superior vena cava were ligated, and a weep hole was done Heart was harvested after a perfusion of 0.5 L cold St Thomas n ° 2 cardioplegia in the ascending aorta. In brief, farm pigs weighing 20-25 kg were used The details of the preparation have been described previously (3). (0.5 M Dotarem, Guerbet, Aulnay sous Bois, France) was For comparison, the extracellular fluid-type contrast agent Gd-DOTA meglumine The polymer, measured by assaying Gd (III), were approximately five times higher than those of Gd-DOTA Overall characteristics of a blood pool agent: more thanģ0% of the injected dose remained in the blood after 30 Iodinated nonionic dimeric contrast agent, but with the The viscosity obtained at this concentration is similar to that of As its intrinsic viscosity (8.5 mPa/s at 37☌) is greater than that of extracellular fluid-type gadolinium chelates, CMD-A2-GdDOTA was prepared as a 0.1 mol Gd/L solution. The paramagnetic efficiency of CMD-A2-Gd-DOTA is approximately three
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The relaxivities in water measured at 20 MHz, 37☌Īnd pH of 7.4 were 10.59 mmol -j s-1 + 0.15 for R1 andġ1.14 m m o k I s-1 + 0.28 for R2. Polymer, substituted with the paramagnetic macrocyclicĬomplex Gd-DOTA with an average molecular weight of Intravascular contrast agent to study myocardial perfusion with MRI.ĬMD-A2-Gd-DOTA is a carboxymethyl-dextran Pig heart preparation and was demonstrated as a potential Gd-DOTA (2) (Laboratoire Guerbet, Aulnay sous Bois,įrance) was evaluated using an isolated blood-perfused Substituted with the paramagnetic macrocyclic complex A prototypical carboxymethyl-dextran polymer Macromolecular-based paramagnetic contrast mediaĬould be suggest ed as a potential blood-pool contrastĪgent. Supported in part by a grant from Laboratoire Guerbet and by a grant from Association Grampri. Casali, M.J., J.F.) and Laboratoire Guerbet, Auinay-sousbois, France (S.B., C. Casali, M.J., E.C., D.R.) Cermep, Lyon,įrance (C. Throughout the vascular and interstitial spaces in the steadyġFrom the Laboratoire Creatis (UMR CNRS 5515), Hopital Cardiologique, 69394 Lyon, France (C. Reflects the passage of a purely vascular indicator.Ĭurrently available paramagnetic MR contrast agentsįor clinical purposes exit the vascular space and distribute One of the major assumptions in nearlyĪll approaches is that the myocardial intensity-time curve Have been demonstrated in animals (1) and in humans withĮither echo-planar imaging (EPI) or gradient-recalled-echo The feasibility of determining myocardial perfusion using MRI and the first-pass techniques Soluble contrast agent (iodinated, paramagnetic, susceptibility contrast agent) during its first pass through the coronary microcirculation. These techniques rely onĬhanges in the myocardial concentration-time curve of a Of contrast to be observed and parameters related to myocardial perfusion to be derived.
![dextran mr contrast dextran mr contrast](https://patentimages.storage.googleapis.com/WO2011122770A9/WO-DOC-FIGURE-1a.png)
Rapid imaging with acquisition onceĮach cardiac cycle allows the myocardial transit of a bolus More recently, ultrafast magnetic resonance imaging acquisition combined with the injection of various classes of Planar digital techniques, fast computed tomography, and, Relative and/or absolute myocardial perfusion using x-ray Several approaches have been evaluated for quantifying Jean Frangois Obadia, MD, PhD, Soraya Benderbous, PhD Cendrine Casali, BS, Marc Janier, MD, Emmanuelle Canet, DVM, PhD